Commercial production of proteases in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

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435 691, 435 698, 435412, 435419, 435468, 800287, 800288, 800295, 800298, 800306, 8003201, A01H 510, A01H 500, C12N 504, C12N 1557, C12N 1512, C12N 1582

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060875585

ABSTRACT:
Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

REFERENCES:
patent: 5097025 (1992-03-01), Benfey et al.
patent: 5122594 (1992-06-01), Yoshida et al.
patent: 5215912 (1993-06-01), Hoffman
patent: 5270200 (1993-12-01), Sun et al.
patent: 5290924 (1994-03-01), Last et al.
patent: 5358934 (1994-10-01), Borovsky et al.
patent: 5424200 (1995-06-01), McPherson et al.
patent: 5457066 (1995-10-01), Frank et al.
patent: 5504200 (1996-04-01), Hall et al.
patent: 5543501 (1996-08-01), Slingtom et al.
patent: 5552527 (1996-09-01), Godlard et al.
patent: 5629196 (1997-05-01), Borovsky et al.
patent: 5693520 (1997-12-01), Branner
patent: 5714580 (1998-02-01), Brunck et al.
patent: 5741664 (1998-04-01), Ballinger et al.
Wyatt, Paul et al. "Correct processing of the kiwifruit protease actinidin in transgenic tobacco requires the presence of the C-terminal propeptide" Plant Physiol. 108(1), 261-8 (1995).
van der Geest, A. and Hall, T. "A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer" Plant Molecular Biology 32:579-588 (1996).
Christensen et al, Plant Mol. Biol., vol. 18, pp. 675-689, 1992.
Odell et al, Nature, vol. 313, pp. 810-812, 1985.
Rogers et al, J. Biol. Chem., vol. 258, pp. 8169-8174, 1983.
Schroeder et al, J. Biol. Chem., vol. 243, pp. 2943-2949, 1968.
Altenbach et al, Plant Mol. Biol., vol. 18, pp. 235-245, 1992.
Wim J. M. Van de Wen,et al., "Structure and Function of Eukaryotic Proprotein Processing Enzymes of the Subtilisin Family of Serine Proteases", Critical Reviews in Oncogenesis 4(2) 115-136 1993.
Takagi, Hiroshi, et al., "Enhancement of the Thermostability of Subtilisin E by Introduction of a Disulfide Bond Engineered on the Basis of Structural Comparison with a Thermophilic Serine Protease", The Journal of Biological Chemistry vol.265, No.12 pp. 6874-6878, Apr. 25, 1990.
Svendsen,I.B, "Chemical Modifications of The Subtilisins With Special Reference to the Binding of Large Substrates, A Review", Carlsberg Res. Commun. vol. 41, No. 5, 1976.
Pierce, Jeffrey A., et al., "Physiological and Genetic Strategies for Enhanced Subtilisin production by Bacillus subtilis", Biotechnol. Prog., vol. 8, pp. 211-218, 1992.
Wells, James A., et al., "Subtilisin-an enzyme designed to be engineered", Trends In Biochemical Sciences, Aug. 1988.
Hiei, Yukoh, et al., "Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA", The Plant Journal 6 (2), pp. 271-282 Jan. 1994.
Ishida, Yuji, et al., "High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens", Nature biotechnology vol. 14, pp. 745-750, Jun. 1996.
Cheng, Ming, et al., "Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens", Plant Physiol p. 971, 1997.
Bustos, Mauricio M., "Regulalion of B-Glucuronidase Expression in Transgenic Tobacco Plants by an A/T-Rich, cis-Acting Sequence Found Upstream of a French Bean B-Phaseolin Gene", The Plant Cell, vol. 1, Sep. 1989.

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