Combining mutations for stabilization of subtilisin

Details Combining mutations for stabilization of subtilisin Combining mutations for stabilization of subtilisin

1988-04-12

1991-02-05

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351723, 435221, 25217412, 935 10, 935 14, 935 29, C12N 1500, C12N 934, C12N 954, C12N 956

Patent

active

049904523

ABSTRACT:
The invention relates to modified subtilisin enzymes which have increased thermal stability. The modified subtilisin enzymes have at least two or more amino acid mutations which confer increased thermal stability. It has been discovered that combining individual stabilizing mutations in subtilisin frequently results in an additive increase in thermal stability. In addition, the invention pertains to cloned mutant genes coding for a subtilisin material having at least two amino acid substitution which has increased thermal stability.

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