Combined radioimmunoassay for triiodothyronine and thyroxine

Details Combined radioimmunoassay for triiodothyronine and thyroxine Combined radioimmunoassay for triiodothyronine and thyroxine

1978-11-20

1980-09-30

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 232306, 424 12, G01N 3316

Patent

active

042255767

ABSTRACT:
A method for the combined radioimmunoassay for the hormones triiodothyronine (T-3) and thyroxine (T-4) in serum or plasma, wherein the radiolabeled T-3 and T-4 reagents are T-3 and T-4 labeled with the same radioisotope. The hormones are extracted from the serum or plasma sample and separated from their binding proteins by adsorption to a crosslinked dextran gel at a highly alkaline pH. After washing away the dissociated proteins, a predetermined limiting amount of T-3 antibody is incubated with the gel and thereafter radiolabeled T-3 which has become extracted from the gel by antibody binding is washed away. Excess T-3 antibody and a predetermined limiting amount of T-4 antibody are then incubated with the gel and radiolabeled T-3 and T-4 which has become extracted from the gel by antibody binding are thereafter washed away. The respective relative amounts of radiolabeled T-3 and T-4 retained by the gel after the incubations with the respective limiting amounts of T-3 and T-4 antibody are compared with standard results to quantitate the hormones in the sample. The use of excess T-3 antibody in the T-4 antibody incubation is critical to the accuracy of the method, enabling the use of the same radioisotope for radiolabeling T-3 and T-4.

REFERENCES:
patent: 3659104 (1972-04-01), Gross et al.
patent: 3911096 (1975-10-01), Chopra
patent: 3928553 (1975-12-01), Holland
patent: 3952091 (1976-04-01), Grunberg et al.
patent: 3961894 (1976-06-01), Gordon et al.
L. J. Unggren et al., Acta. Endocrinol., 81:487-494 (1976).
Haberman et al. J. Clin. Chem. Clin. Biochem., 14:495-601 (1976).

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