Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus
Reexamination Certificate
2001-10-19
2008-07-01
Tsang, Cecilia (Department: 1654)
Chemistry: molecular biology and microbiology
Apparatus
Mutation or genetic engineering apparatus
Reexamination Certificate
active
07393680
ABSTRACT:
Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed between said needle-shaped object, with the tip of said needle-shaped object placed in contact with said conainer in such a way that it applies a mechanical force to the lipid membrane of said container, thus mechanically straining it, and a second electrode, whereupon a transient electric pulse of 1-to-103V/cm is applied between the electrodes, resulting in a focused electrical field over said container C which induces a dielectric breakdown of the lipid bilayer causing the needle-shaped object to penetrate the container. Disclosed is also an electroinjection method based on the above method, wherein substances are introduced through the needle-shaped object and into the container after penetration of the needle-shaped object.
REFERENCES:
patent: 4784737 (1988-11-01), Ray et al.
patent: 5859327 (1999-01-01), Dev et al.
patent: 5874268 (1999-02-01), Meyer
patent: 6079230 (2000-06-01), Kong
patent: 6261815 (2001-07-01), Meyer
patent: 2002/0061589 (2002-05-01), King et al.
patent: 2002/0062126 (2002-05-01), Lewis et al.
patent: 2002/0127723 (2002-09-01), Palermo
patent: 2002/0133137 (2002-09-01), Hofmann
patent: 2002/0160437 (2002-10-01), Meyer
patent: 2002/0198485 (2002-12-01), Dev et al.
patent: 2002/0198512 (2002-12-01), Seward
patent: 2003/0009113 (2003-01-01), Olson
patent: 2003/0009148 (2003-01-01), Hayakawa
patent: 2003/0017598 (2003-01-01), Burke et al.
patent: 19950133907 (1995-05-01), None
patent: WO 99/24110 (1999-05-01), None
patent: WO 00/34434 (2000-06-01), None
patent: WO 01/07583 (2001-01-01), None
patent: WO 01/48180 (2001-05-01), None
patent: WO 02/31171 (2002-04-01), None
patent: WO 02/100459 (2002-12-01), None
Karlsson, et al., “Electroinjection of Colloid Particles and Biopolymers into Single Unilamellar Liposomes and Cells for Bioanalytical Applications”, Anal. Chem., 72, 5857-5862 (2000).
Swartz, et al., “Sparking New Frontiers: Using in Vivo Electroporation for Genetic Manipulations” Development Biology 233, 13-21 (2001).
Lundqvist, et al., “Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes”, Proc. Natl. Acad. Sci. USA, vol. 95, pp. 10356-10360, Sep. 1998.
Needham, et al., “Electro-mechanical permeabilization of lipid vesicles, Role of membrane tension and compressibility”, Biophys J. ® Biophysical Society, vol. 55, May 1989, 1001-1009.
Wick, et al., “Microinjection into giant vesicles and light microscopy investigation of enzyme-medicated vesicle transformations”, Chemistry & Biology, Feb. 1996, 3:105-11.
Clark, et al., “Optical Nanosensors for Chemical Analysis inside Single Living Cells. 1. Fabrication, Characterization, and Methods for Intracellular Delivery of Pebble Sensors”, Anal. Chem., 1999, 71, 4831-4836.
Chiu Daniel
Karlsson Mattias
Orwar Owe
Bradley Christina Marchetti
Cellectricon AB
Conlin David G.
Edwards Angell Palmer & & Dodge LLP
Kopacz Jeffrey L.
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