Combined electrophoresis-electrospray interface and method

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, 250288, G01N 2726, G01N 27447, H01J 4904

Patent

active

054239648

ABSTRACT:
An improvement to the system and method for analyzing molecular constituents of a composition sample that comprises improvements to an electrospray ionization source for interfacing to mass spectrometers and other detection devices. The improvement consists of establishing a unique electrical circuit pattern and nozzle configuration, a metallic coated and conical shaped capillary outlet, coupled with sizing of the capillary to obtain maximum sensitivity.

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patent: 5115131 (1992-05-01), Jorgenson et al.
Richard D. Smith, Charles J. Barinaga, and Harold R. Udseth, "Improved Electrospray Ionization Interface for Capillary Zone Electrophoresis-Mass Spectrometry" Analytical Chemistry vol. 60 No. 18, (Sep. 1988) 1948-1952.
Richard D. Smith, Harold R. Udseth, Charles J. Barinaga, Charles G. Edmonds "Instrumentation for high-performance capillary electrophoresis-mass spectrometry" Journal of Chromatography, 559 (1991) 197-208.
"Attomole Level Capillary Electrophoresis-Mass Spectrometric Protein Analysis Using 5 .mu.m i.d. Capillaries", J. H. Wahl, D. R. Goodlett, H. R. Udseth, R. D. Smith, Anal. Chem., 64, 3194-3196 (Dec. 1992).
"Use of Small-Diameter Capillaries for Increasing Peptide and Protein Detection Sensitivity in Capillary Electrophoresis-Mass Spectrometry," J. H. Wahl, D. R. Goodlett,H. R. Udseth, R. D. Smith, Electrophoresis, 14, 448-457 (1993).
"Capillary Electrophoresis-Mass Spectrometry," R. D. Smith, J. H. Wahl, D. R. Goodlett, and S. A. Hofstadler, Anal. Chem., 65, 574A-584A (Jul. 1993).
"Sheathless Capillary Electrophoresis-Electrospray Ionization/Mass Spectrometry using 10 .mu.m i.d. Capillaries: Analysis of Tryptic Digests of Cytochrome c," J. H. Wahl, D. C. Gale, and R. D. Smith, J. Chromatography.

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