Combinatorial libraries and methods for their use

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436501, 436518, 436528, 436531, G01N 3353

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active

058076832

ABSTRACT:
Kit, and method for its use and construction, which includes a first plurality of vessels containing different polysubunits, each constructed from a known number of subunits. Each subunit is joined by one or more bonds and each subunit and bond can be the same or different. Each of the different polysubunits has a different sequence of subunits but has the same known subunit at one terminus. Each vessel contains polysubunits which have a different known subunit at one terminus. The kit further includes a second plurality of vessels which includes different polysubunits, each constructed from the known number minus one subunit, as described above.

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Furka et al "More Peptides by Less Labow" Eur. Fed. Med. Chem., 8.sup.th International Symposium on Med. Chem. p.-168 (1988).
Greysen, et al., Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid, Proc. Natl. Acad. Sci. USA, vol. 81, pp. 3998-4002, Jul. 1984.
Lam et al., A new type of synthetic peptide library for identifying ligand-binding activity, Nature, vol. 354, 7 Nov. 1991, pp. 82-84.
Houghten et al., Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery, Nature, vol. 354, 7 Nov. 1991, pp. 84-86.

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