Combinational usage of insulators and coat color marker for...

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of dna into an embryo – egg cell – or...

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C435S320100

Reexamination Certificate

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07067715

ABSTRACT:
A method of identifying a genotype of a transgenic mouse and reducing the variability of a transagene expression in the transgenic mouse comprises the step of introducing into a mouse (a) a transgene expression cassette, (b) a coat color expression cassette and (c) an insulator positioned at 5′ or 3′ end of the transgene expression cassette.

REFERENCES:
Niemann H “Transgenic farm animals get off the ground.” Transgenic Research 7:73-75, 1997.
Kappel et al. “Regulation gene expression in transgenic animals.” Current Opinion in Biotechnology 3:548-553, 1992.
Sigmund CD “Viewponit: are studies in genetically altered mice out of control?” Arterioscler Thromb Vasc Biol 20:1425-1429, 2000.
Cameron ER “Recent advances in transgenic technology” Molecular Biotechnology 7:253-265, 1997.
Wall RJ “New gene transfer methods.” Theriogenology 57:189-201, 2002.
Mullins et al, “Transgenesis in nonmurine species.” Hypertension 22:630-633, 1993.
Wang et al. “Isolation, cloning and sequencing of porcine agouti exon 2 (PorAex2).” Pigment Cell Res 11(3):155-157, 1998.
Manne et al. “Mechanisms for the pleiotropic effects of the agouti gene,” Proc Natl Acad Sci 92:4721-4724, 1995.
Ahearn JM, Cloning and sequence analysis of the mouse genomic locus encoding the largest subunit of RNA polymerase II, JBC, 1987, vol. 262, pp. 10695-10705.
Scheider MR, Transgenic mouse models for studying the functions of insulin-like growth factor-binding proteins, FASEB, 2000, vol. 14, pp. 629-640.
Gotz J, In vivo analysis of wild-type and FTPD-17 tau transgenic mice, Annal of NY Acad of Sci, 2000, vol. 920, pp. 126-133.
A. C. Bell, “The Protein CTCF is Required for the Enchancer Blocking Activity of Vertebrate Insulators”, Cell, vol. 98, 387-396, Aug. 6, 1999.
A. C. Bell, “Insulators and Boundaries: Versatile Regulatory Elements in the Eukaryotic Genome”, Science, vol. 291, Jan. 19, 2001.
J. H. Chung et al., “Characterization of the Chicken β-globin Insulator”, Proc. Natl. Acad. Sci., vol. 94, pp. 575-580, Jan. 1997.
K. Dobie et al., “Variegated Gene Expression in Mice”, TIG, Apr. 1997, vol. 13, No. 4.
D. M. J. Duhl et al., “Pleiotropic Effects of the MouseLethal Yellow(Ay) Mutation Explained by Deletion of a Maternally Expressed Gene and the Simultaneous Production ofagoutiFusion RNAs”, Development 120, 1695-1708 (1994).
D. W. Emery, et al., “Chromatin Insulator Protects Retrovirus Vectors From Chromosomal Position Effects”, Proc. Natl. Acad Sci., Aug. 1, 2000, vol. 97 No. 16, pp. 9150-9155.
T. I. Gerasimova et al., “Chromatin Insulators and Boundaries: Effects on Transcription and Nuclear Organization”, Annu. Rev. Genet. 2001, 35: 193-208.
M. J. Pikaart et al., “Loss of Transcriptional Activity of a Transgene is Accompanied by DNA Metylation and Histone Deacetylation and is Prevented by Insulators”, Genes & Development 12:28553-2862.
M. N. Prioleau et al., “An Insulator Element and Condensed Chromatin Region Separate the Chicken β-globin Locus from an Independently Regulated Erythroid-Specific Folate Receptor Gene”, The EMBO Journal, vol. 18, No. 14, pp. 4035-4048, 1999.
F. Recillas-Targa et al., “Positional Enhancer-Blocking Activity of the Chicken β-globin Insulator in Transiently Transfected Cells”, Proc. Nat'l Acad. Sci., vol. 96, No. 25, pp. 14354-14359.
T. F. Tsai et al., “Paternal Delection fromSnrpntoUbe3ain the Mouse Causes Hypotoni, Growth Retardation and Partial Lethality and Provides Evidence for a Gene Contributing to Prader-Willi Syndrome”, Human Molecular Genetics, 1999, vol. 8, No. 8, 1357-1364.
T. F. Tsai, et al., “Evidence for Translational Regulation of the ImprintedSnurgf-Snrpnlocus in Mice”, Human Molecular Genetics, 2002, vol. 11, No. 14, 1659-1668.

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