Liquid purification or separation – Serially connected distinct treating with or without storage... – Diverse
Patent
1987-05-14
1988-09-13
Schor, Kenneth M.
Liquid purification or separation
Serially connected distinct treating with or without storage...
Diverse
210317, 21032172, 422 44, 436518, B01D 1300
Patent
active
047707747
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to a column for removing .beta..sub.2 -microglobulin. More particularly, this invention relates to a column which specifically adsorbs and removes .beta..sub.2 -microglobulin in the blood.
BACKGROUND ART
.beta..sub.2 -microglobulin is a light chain of a double-stranded protein constituting the major histocompatibility antigen (in case of human, it is HLA, class I), and occurs on the surfaces of most of cells. It also occurs in the body fluid in the free form, but the physiological function of the free .beta..sub.2 -microglobulin has not yet been known. The full amino acid sequence thereof has been determined for human and other various animals, and its three dimensional structure has been determined by X-ray analysis for bovine. It has been proved that it is a simple protein with a molecular weight of about 12,000, which does not have a sugar chain, and that it has structurally high homology with the C domain (constant domain) of immunogulobulin. Further, the homology of the amino acid sequence thereof between different species is 60 to 80%, and thus it is considerably high (Proc. Natl. Acad. Sci. 257, 2619 (1982)).
The .beta..sub.2 -microglobulin level in the blood of the patients suffering from nephropathy, who are undergoing artificial blood dialysis for a long period, is as high as 10 to 100 times that of normal human. It is assumed that this is because that .beta..sub.2 -microglobulin which is decomposed in the kidney is not removed by the blood dialysis and thus accumulates in the blood.
The present inventors separated and analyzed the amyloid proteins deposited on the diseased part of a patient suffering from carpal tunnel syndrome to find that most of the amyloid proteins are .beta..sub.2 -microglobulin. Thus, it is assumed that carpal tunnel syndrome is caused by the deposition of the .beta..sub.2 -microglobulin on the diseased part, which is accumulated in the blood with high level. Thus, it is expected that carpal tunnel syndrome can be prevented by removing the .beta..sub.2 -microglobulin in the blood along with the artificial blood dialysis. Further, it is possible that .beta..sub.2 -microglobulin is involved in the deposition of amyloid on the parts other than the carpal tunnel.
Heretofore, no disease has been known of which cause is clarified to be the .beta..sub.2 -microglobulin in the blood, and so how to remove the .beta..sub.2 -microglobulin in the blood has not been considered at all.
DISCLOSURE OF THE INVENTION
The object of the present invention is to provide a method for selectively removing .beta..sub.2 -microglobulin in the blood.
The object can be accomplished by the present invention which provides a column for adsorbing and removing .beta..sub.2 -microglobulin, which employs immobilized anti-.beta..sub.2 -microglobulin antibody.
BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1(a) and 1(b) show examples of circuits which employs blood dialyzer and .beta..sub.2 -microglobulin-removing column together, wherein FIG. 1(a) shows an example in which they are connected in series, and FIG. 1(b) shows an example in which they are connected in parallel;
FIG. 2 is a schematic view showing the results of SDS-polyacrylamide gel electrophoresis of column fractions obtained in Example 1;
FIG. 3 shows the total amount of proteins and the level of .beta..sub.2 -microglobulin of the column fractions obtained in Example 2;
FIGS. 4(a), 4(b) and 4(c) show the change in time of the amount of the remaining .beta..sub.2 -microglobulin in the blood, wherein 4(a) shows the results obtained by using a column in which anti-.beta..sub.2 -microglobulin antibody is immobilized, 4(b) shows the results obtained by reusing the column of (a), and 4(c) shows the results obtained by using a column in which anti-.beta..sub.2 -microglobulin is not immobilized.
BEST MODE FOR CARRYING OUT THE INVENTION
Both of a polyclonal antibody which is obtained by immunizing animals such as mice, rats, rabbits, goats and sheep, and a monoclonal antibody obtained by using a cell hybridiza
REFERENCES:
patent: 3669878 (1972-06-01), Marantz
patent: 4257884 (1981-03-01), Lim
patent: 4321192 (1982-03-01), Jain
patent: 4329152 (1982-05-01), Lauwerys
patent: 4569919 (1986-02-01), Toth
Termin, FEBS Letters, vol. 61, No. 1, (Jan. 1976), pp. 59-62, North-Holland Pub. Co.-Amsterdam.
Sevier, Clin. Chem., vol. 27, No. 11, (1981), 1797-1802.
Ida Nobuo
Kataoka Hiroshi
Kunitomo Tetsunosuke
Miller Austin R.
Schor Kenneth M.
Toray Industries Inc.
LandOfFree
Column for removing .beta..sub.2 -microglobulin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Column for removing .beta..sub.2 -microglobulin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Column for removing .beta..sub.2 -microglobulin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-805470