Column for capillary chromatographic separations

Details Column for capillary chromatographic separations Column for capillary chromatographic separations

1997-10-10

1999-06-01

Therkorn, Ernest G.

Liquid purification or separation

With means to add treating material

Chromatography

210656, 96101, 204601, B01D 1508

Patent

active

059085525

ABSTRACT:
A column for capillary chromatographic separations, for example high performance liquid chromatography, capillary electrochromatography, or supercritical chromatography, includes a column bed of packing material arranged in the inner bore of the column, and a retainer for retaining the column bed in the interior of the column, wherein the retainer includes regions in the interior of the column having radial dimensions which are different from that of the inner bore. Preferably, the column is a fused silica capillary whose protective layer is removed in a detection area so that sample substances separated in the column can be detected by means of a light source which transmits radiation through the column which is detected by a detector.

REFERENCES:
patent: 3782078 (1974-01-01), Jerpe
patent: 4293415 (1981-10-01), Bente
patent: 4793920 (1988-12-01), Cortes
patent: 5061361 (1991-10-01), Gordon
patent: 5246577 (1993-09-01), Fuchs et al.
European Search Report, Jun. 26, 1996, EP 95 11 9699, pp. 1-4.
Analytical Methods and Instruments, vol. 2, No. 3, pp. 122-128, Hong Chen et al., "On-Column UV Absorption Detection in Liquid Chromatography with Packed Capillaries", 1995.
Chromatographia, vol. 40, No. 5/6, Mar. 1995, pp. 329-335, R. J. Boughtflower et al., "Capillary Electrochromatography-Some Important Considerations in the Preparation of Packed Capillaries and the Choice of Mobile Phase Buffers".

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