Column apparatus and process for immobilized enzyme reactions

Details Column apparatus and process for immobilized enzyme reactions Column apparatus and process for immobilized enzyme reactions

1976-03-29

1977-06-14

Shapiro, Lionel M.

Textiles: fiber preparation

195 31F, 195 63, 195 68, 195116, 195139, 195DIG11, C12D 1302, C12B 100, C07G 702

Patent

active

040295460

ABSTRACT:
A column apparatus and process is provided for immobilized enzyme reactions using a bed of regenerated sponge material chemically modified to incorporate enzyme-immobilizing groups. The sponge material selected for use in column apparatus has a flow of direction length of 2 to 5 feet or more, a density of less than 1 gram for each 18 cc. of bed volume (e.g. 1 g./20-70 cc. bed vol.), and a water flow porosity such that over 0.5 (preferably over 1.0) gal. water/min./ft..sup.2 flows through the bed. The apparatus and process can be used with a wide variety of enzymes, but has particular applicability to the enzymatic conversion of starch hydrolysates. Starch-derived sugar syrups having D.E.'s of 40 to 97 can be produced. Also, isomerization of glucose syrups can be carried out to produce a syrup of enhanced sweetness, comprising a mixture of glucose and fructose.

REFERENCES:
patent: 3919048 (1975-11-01), Dahlmans et al.
patent: 3944470 (1976-03-01), Diehl et al.
patent: 3960663 (1976-06-01), Tamura et al.

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