Colormetric method for quantification of magnesium using .alpha.

Details Colormetric method for quantification of magnesium using .alpha. Colormetric method for quantification of magnesium using .alpha.

1986-07-03

1989-04-04

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 25, 435 28, C12Q 148, C12Q 126

Patent

active

048186910

ABSTRACT:
A method and composition for determining magnesium levels in a liquid. The composition contains a first enzyme reagent part having glycerol-kinase, adenosine 5'-triphosphate, 4-aminoantipyrene, .alpha.-glycerophosphate oxidase, peroxidase, glycerol kinase, water and a buffer capable of maintaining the solution at a pH between about 5.5 and about 9.2. The composition also contains a second trigger reagent part which has sodium 2-hydroxy-3,5-dichlorobenzene sulfonate, glycerol, water and a buffer capable of maintaining the solution pH between about 5.5 and about 9.2. In the method of the present invention, a liquid to be analyzed is contacted sequentially with the first enzyme reagent part and followed by the second trigger reagent and is measured to quantitatively detect the presence of a detectable species produced as a result of the reactions triggered by the mixture of the enzyme reagent part and the trigger reagent part.

REFERENCES:
patent: 4241178 (1980-12-01), Esders et al.
patent: 4547461 (1985-10-01), Esders et al.
patent: 4657854 (1987-04-01), Wegfahrt
Clinical Chemistry, vol. 32/4, pp. 629-632, "A Kinetic Colorimetric Procedure for Quantifying Magnesium in Serum", Wimmer et al.

Affiliated with

Also associated with

Rating

Colormetric method for quantification of magnesium using .alpha. does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Colormetric method for quantification of magnesium using .alpha., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Colormetric method for quantification of magnesium using .alpha. will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-180184