Colorimetric monitoring of reactions in solution and solid...

Details Colorimetric monitoring of reactions in solution and solid... Colorimetric monitoring of reactions in solution and solid...

2006-04-13

2010-11-30

Siefke, Sam (Department: 1797)

Chemistry: analytical and immunological testing

Optical result

Including reagent preparation

C436S164000, C436S800000, C422S082060, C422S082050

Reexamination Certificate

active

07842511

ABSTRACT:
The present invention relates to the a new protecting group, 4-(O-nitrophthalimido)butyric acid (NPB) that may be attached to a monomer unit during oligosaccharide synthesis such that upon cleavage of this molecule, the solution turns orange. The orange color allows the simple colorimetric monitoring of the completion of the cleavage reaction and, upon collection of the colored fraction, quantification of the amount of the monomer unit that was successfully coupled to the solid phase.

REFERENCES:
patent: 5512668 (1996-04-01), Stec et al.
patent: 7135565 (2006-11-01), Dellinger et al.

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