Colorimetric method for determining total lipids in human fluids

Details Colorimetric method for determining total lipids in human fluids Colorimetric method for determining total lipids in human fluids

1975-05-19

1977-03-15

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3316, G01N 2102

Patent

active

040121968

ABSTRACT:
Phospho-vanillin reagent suitable for reaction with a mixture of sulfuric acid and an animal fluid containing at least 1 mg/ml lipids to determine total lipid concentration in the animal fluid comprising an aqueous solution containing vanillin and phosphoric acid having a vanillin to phosphoric acid mole ratio between 1.12 .times. 10.sup.-.sup.3 and 2.08 .times. 10.sup.-.sup.3. A sample of the animal fluid is mixed with concentrated sulfuric acid in a container, incubated, and the phospho-vanillin reagent is added to the mixture in the same container to form a chromogen. The light absorbance of the chromogen is measured and the total lipid concentration is determined in accordance with Beer's law from a standard curve relating total lipid concentration as a function of light absorbance by the chromogen.

REFERENCES:
frings & Dunn, Am. J. Clin. Path., v. 53, pp. 89-91 (1970).
Frings et al., Clin. Chem., v. 18, pp. 673-674 (1972).
Ratliff et al., Advances in Automated Analysis, vol. 1, pp. 101-107 (1972).

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