Colorimetric method for determining gamma-glutamyl transpeptidas

Details Colorimetric method for determining gamma-glutamyl transpeptidas Colorimetric method for determining gamma-glutamyl transpeptidas

1977-01-12

1978-05-02

Tanenholtz, Alvin E.

Textiles: fiber preparation

1951035R, G01N 3300, G01N 3114

Patent

active

040873315

ABSTRACT:
A colorimetric method for the determination of gamma-glutamyl transpeptidase enzyme activity in a biological fluid comprises catalytically reacting the enzyme in the fluid with a substrate of a water-soluble salt of a compound selected from the group consisting of L-gamma-glutamyl-3-sulfoanilide and L-gamma-glutamyl-4-sulfoanilide in aqueous solution to produce the corresponding aniline sulfonic acid salt as a cleavage product, diazotizing the cleavage product, and coupling the diazotized product with a coupling compound to produce a dye having a color intensity proportional to the enzyme activity.

REFERENCES:
patent: 3892631 (1975-07-01), Carroll
patent: 3986931 (1976-10-01), Bernt et al.
Goldbarg et al. Arch. Biochem. and Biophys., 91, (1960) pp. 61-70.

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