Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-n
Patent
1992-12-24
1993-12-14
Housel, James C.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-n
436111, 436171, 436813, 356319, G01N 3300, G01N 2177, G01J 342
Patent
active
052702154
ABSTRACT:
A spectrophotometric assay for quantitative determination of 5-hydroxyindoleacetic acid in fluid samples. 3-methyl-2-benzthiazolone hydrazone is oxidized with sodium periodate to the corresponding diazonium salt. The resulting diazonium salt adds electrophilically to activated positions on indole ring of 5-hydroxyindoleacetic acid to yield azo dyes which absorb light at 510 nm.
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patent: 4863873 (1989-09-01), Matson
patent: 5104639 (1992-04-01), Matson
Goldenberg; "Specific Photometric Determination of 5-Hydroxyindoleacetic Acid in Urine", Clin. Chem., 19, 38 (1973).
Mukerjee, et al.; "A Colorimetric Determination of 5-Hydroxyindole Acetic Acid in Urine", Clin. Chem. Acta, 209, 105 (1992).
Sawicki, et al.; "Spot Test Detection and Colorimetric Determination of Aromatic Amines and Imino Heteroaromatic Compounds with 3-Methyl-2-benzthiazolone Hydrazone", Anal. Chem., 32, 722 (1961).
Undenfriend, et al.; "The identification of 5-Hydroxy-3-indoleacetic acid in Normal Urine and a Method for its Assay", J. Biol. Chem., 216, 499-505 (1955).
Mukerjee Harry
Pincus Matthew R.
Bhat N.
Housel James C.
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