Colorimetric detection of delta-5-3-ketosteroid isomerase and im

Details Colorimetric detection of delta-5-3-ketosteroid isomerase and im Colorimetric detection of delta-5-3-ketosteroid isomerase and im

1987-03-13

1989-07-11

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435810, G01N 33535, C12Q 100

Patent

active

048471941

ABSTRACT:
A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, an antianalyte and a tracer having conjugated thereto delta 5-3-ketosteroid isomerase. A bound fraction is separated from the liquid and incubated in a second liquid with a delta 5-3-ketosteroid substrate. Isomerase on the bound fraction converts the substrate to the corresponding delta 4-3-ketosteroid. A tetrazolium salt and a base are added. Unconverted delta 5-3-ketosteroid reduces the tetrazolium salt to a formazan which imparts a color to the liquid. A signal associated with the color may be used to detect or to measure the concentration of the analyte in the liquid. The invention includes a kit of materials useful in performing the assay of the invention.

REFERENCES:
patent: 3654090 (1972-04-01), Hermans et al.
patent: 4298686 (1981-11-01), Nicholas et al.
patent: 4350760 (1982-09-01), Nicholas et al.
patent: 4769321 (1988-09-01), Self
Obuyama, Chemical Abstract, 87 (1977), abstract number 201867h.
Meyer et al, Anal. Chem., 27, 813-817, (1955).
Graham et al, J. Pharm. Science 64 (1975) 226-230.

Affiliated with

Also associated with

Rating

Colorimetric detection of delta-5-3-ketosteroid isomerase and im does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Colorimetric detection of delta-5-3-ketosteroid isomerase and im, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Colorimetric detection of delta-5-3-ketosteroid isomerase and im will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-436917