Colorimetric biological assay

Details Colorimetric biological assay Colorimetric biological assay

1984-11-29

1989-07-18

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 29, 435 34, 435 39, C12Q 132, C12Q 102, C12Q 104, C12Q 106

Patent

active

048493473

ABSTRACT:
Lactic acid produced by cells in culture is detected and used in a bioassay to determine cell growth and vitality. Lactic acid is detected by coupling the lactate dehydrogenase conversion of lactic acid to pyruvic acid to a reaction using micotinamide adenine dinucleotide in reduced form (NADH) to reduce a tetrazolium dye. The process can be used to serve as a bioassay for measuring biologically active compounds, particularly lymphokines.

REFERENCES:
patent: 3326777 (1967-06-01), Babson
patent: 3910824 (1975-10-01), Cartwright et al.
patent: 4003795 (1977-01-01), Lamprecht
Alberts et al., Molecular Biology of the Cell (1983), Garland Publishing, Inc., N.Y., pp. 523 and 996.
Carpenter, Microbiology, 3rd Ed. (1972), W. B. Saunders Co., Philadelphia, pp. 258-259.
Klebe, "In Vitro", vol. 20, No. 2 (Feb. 1984), pp. 127-132.
Zolg et al., "In Vitro", vol. 20, No. 3 (Part I), (Mar. 1984), pp. 205-215.

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