Colorimetric assay for bilirubin

Details Colorimetric assay for bilirubin Colorimetric assay for bilirubin

1977-01-14

1978-01-17

Reese, Robert M.

Chemistry: physical processes

Physical processes

Crystallization

23253TP, G01N 3316

Patent

active

040690172

ABSTRACT:
A colorimetric assay method for the detection of bilirubin in various liquid samples, particularly biological liquids. A multi-zone analytical element based on this assay method is also disclosed. The assay method employs an interactive mordant composition for bilirubin having one or more binding sites for bilirubin, such composition comprising a hydrophobic organic matrix and at least one charge-bearing cationic group. Upon mordanting of bilirubin by the interactive mordant composition, the mordanted bilirubin exhibits an absorption peak shift of at least 10 nm. and at least a 50% increase in molar extinction coefficient.

REFERENCES:
patent: 2854317 (1958-09-01), Free et al.
patent: 3754862 (1973-08-01), Wahlefeld et al.
patent: 3814586 (1974-06-01), Fraser, Jr. et al.
patent: 3853476 (1974-12-01), Rittersdorf et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.

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