Colorimetric assay by enzymatic oxidation in the presence of an

Details Colorimetric assay by enzymatic oxidation in the presence of an Colorimetric assay by enzymatic oxidation in the presence of an

1989-08-09

1993-04-27

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, C12Q 126, C12Q 132

Patent

active

052061477

ABSTRACT:
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of the reduced electron acceptor by color formation as measure for the amount of the analyte, wherein the analyte is oxidized with an appropriate flavine- or PQQ-dependent oxidoreductase in the presence of aromatic nitroso or oxime compounds. The present invention also provides an agent for the colorimetric determination of an analyte by enzymatic oxidation of the analyte, containing flavine- or PQQ-dependent oxidoreductase and a color-forming electron acceptor, wherein the color-forming electron acceptor is an aromatic nitroso or oxime compound.

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