Color screening assay for identifying inhibitor resistant HIV pr

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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4353201, 435219, 435 23, 435 24, C12Q 137

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054361311

ABSTRACT:
A chromogenic assay is described for the identification and isolation of drug-resistant HIV protease mutants. Coversely, the assay is useful to screen for new inhibitors of HIV protease, e.g., inhibitors not affected by drug-resistance of the HIV protease. This color screening assay contains a vector comprising a regulatable promoter which controls the transcription of two adjacent structural sequences, one sequence coding for HIV protease or mutant thereof, the other sequence coding for beta-galactosidase with an amino acid substrate insert cleavable by HIV protease. A library of HIV proteases is also described and is isolated in the form of a collection of such vectors, which is a color screen vector library.

REFERENCES:
DBA Abstract, Accession No. 90-03222, Wolf, German Patent No. De 3,819,846.
Kohl et al., Proc. Natl. Acad. Sci. USA, vol. 85, (Jul. 1988), pp. 4686-4690.

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