Color marker in streptomycetes plasmids

Details Color marker in streptomycetes plasmids Color marker in streptomycetes plasmids

1987-08-11

1990-10-16

Martinell, James

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 712, 43525235, 4351723, 935 29, 935 75, C12N 1500, C12N 120, C12P 2100

Patent

active

049634968

ABSTRACT:
Intensely blue-black colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of a streptomycetes recipient strain. Re-isolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the gene for the coloring agent is located. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.

REFERENCES:
patent: 4828998 (1989-05-01), Wohner et al.
Habermehl et al., "Amylocyanin, the Blue Pigment of Streptomyces coelicolor", Chem. Abst., vol. 86, 185565e (1977).
Chater et al; Top. Microbiol. Immunoll 96: 69-95 (1982).
Habermehl et al., "Isolation, Separation and Structure of the Blue Bacterial Pigment . . .", Chem. Abst., vol. 88, 47257w (1978).
Katz, E. et al., "Cloning and Expression of the Tyrosinase Gene from Streptomyces Antibioticus in Streptomyces lividans," J. Gen'l Microbiology 129: 2703-14 (1983).

Affiliated with

Also associated with

Rating

Color marker in streptomycetes plasmids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Color marker in streptomycetes plasmids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Color marker in streptomycetes plasmids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-849632