Chemistry: molecular biology and microbiology – Vector – per se
Patent
1987-08-11
1990-10-23
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 435 712, 435 713, 43525235, 435886, 530 27, 935 11, 935 29, C12N 1500, C12N 120, C12N 100, C12P 2100, C07H 2104
Patent
active
049652079
ABSTRACT:
Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
REFERENCES:
patent: 4828998 (1989-05-01), Wohner et al.
Feitelson et al.; J. Gen. Microbiol. 131: 2431 (1985).
Horinouchi et al.; J. Bacteriol. 155: 1238 (1983).
Piruzyan, et al., "Cloning of DNA Fragments of Streptomyces coelicolor A3(2) Plasmid . . . ", Chemical Abstracts, vol. 98, No. 7, 47927w (1983).
Horinouchi, et al., "Construction and Application of a Promoter-Probe Plasmid . . . ", Journal of Bacteriology, vol. 162, No. 1:406-412 (1985).
Chater, et al., "Gene Cloning in Streptomyces", Curr. Top. Microbiol. Immunol., vol. 96:69-95 (1982).
Feitelson, et al., "Molecular Genetics of Red Biosynthesis in Streptomyces", Journal of Natural Products, vol. 49, No. 6:988-994 (1986).
Katz, E., et al., "Cloning and Expression of the Tyrosinase Gene from Streptomyces antiboiticus in Streptomyces lividans", J. Gen'l Microbiology 129:2703-14 (1983).
Marquardt Rudiger
Muth Gunter
Puhler Alfred
Wohlleben Wolfgang
Wohner Gerhard
Hoechst Aktiengesellschaft
Martinell James
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