Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1984-12-06
1987-06-09
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 14, 435 28, 435805, 435810, 546 23, 436 66, C12Q 162, C12Q 154, C12Q 128, G01N 3372
Patent
active
046720294
ABSTRACT:
Particular heterocyclic compounds are useful as color-forming couplers in analytical compositions, elements and methods. These compounds are represented by the structure: ##STR1## wherein R is a solubilizing group, R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are independently hydrogen, alkyl, alkoxy, aryl, aryloxy, a heterocyclic group, halo, amino or R, and Z represents the carbon atoms necessary to complete the nucleus of a 5- or 6-membered fused ring. The color-forming coupler is combined with an oxidizable color developing compound, e.g. 4-aminoantipyrine to provide a chromogenic composition. This composition can be used in solution or dry assays of biological fluids to produce a color dye in the presence of hydrogen peroxide or other analytes, e.g. glucose or uric acid, which react to produce hydrogen peroxide.
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Cook Ewell R.
Dappen Glen M.
Washburn William N.
Deck Randall E.
Eastman Kodak Company
Tucker J. Lanny
Warden Robert J.
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