Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1981-08-13
1982-11-30
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 11, 435 14, 435 19, 435 25, 435 26, 435 28, 435805, 435810, 23230B, 422 56, C12Q 126, C12Q 128, C12Q 144, C12Q 154, C12Q 160, C12Q 162, C12Q 132
Patent
active
043616488
ABSTRACT:
Analytical element method of making the analytical element and process for determining an analyte in a body fluid sample are disclosed. More particularly, the method of preparing the element comprises the steps of: (a) impregnating a carrier with a first solution, having a pH not greater than about 2.5, which comprises a polymeric mordant and a tetraalkylbenzidine dihydrochloride, in a molar concentration greater than that of the polymeric mordant in an aqueous solvent and drying the carrier; (b) impregnating the carrier of (a) with a solution, having a pH of at least about 7.0, which comprises an analyte-responsive component in an aqueous solvent and drying the carrier. Optionally, there can be added the step of impregnating the carrier of (b) with a solution of a semi-permeable polymer in an organic solvent and drying the carrier.
REFERENCES:
patent: 3298789 (1967-01-01), Mast
patent: 4038485 (1977-07-01), Johnston et al.
patent: 4211845 (1980-07-01), Genshaw et al.
patent: 4273868 (1981-06-01), Walter
patent: 4303753 (1981-12-01), Lam
Coe Roger N.
Kepplinger Esther M.
Miles Laboratories Inc.
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