Color developing method in clinical examinations

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 28, 435 25, G01N 3353, C12Q 128, C12Q 126

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active

049406604

ABSTRACT:
A process for the determination of peroxidase activity or hydrogen peroxide in a clinical sample colorometrically by adding to the sample a reduced form coenzyme, a reagent selected from amines, phenols, and naphthols, a compound which is colored in the reduced form, and peroxidase or an aqueous solution of hydrogen peroxide. The process is essentially unaffected by the presence of ascorbic acid, bilirubin or other reducing substances of body fluids.

REFERENCES:
patent: 4350762 (1982-09-01), De Luca et al.
patent: 4592996 (1986-05-01), Yamanishi et al.
Sevier, E. D., et al., Clin. Chem., vol. 27, No. 11, pp. 1797-1806 (1981).
Michal, G., Mollering, H., Siedel, J., "Chemical Design of Indicator Reactions for the Visible Range", In Methods of Enzymatic Analysis, Third Edition, (Bergmeyer, ed). vol. I (Dec. 1985) pp. 197-221.

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