Colony hybridization method

Details Colony hybridization method Colony hybridization method

1983-08-03

1985-08-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435253, 435259, 435805, 435820, 436 63, 436 94, C12Q 168, C12P 1934, C12N 120, C12N 106

Patent

active

045336288

ABSTRACT:
In the colony hybridization technique, bacterial colonies are replica-plated onto filter paper, and their DNA is hybridized with labeled DNA containing a specific sequence. A one-hundred fold increase in sensitivity is obtained by subjecting the colonies to a stream of steam in the presence of alkali for about three minutes prior to hybridization.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
Grunstein et al., 1975, "Colony Hybridization: A Method for the Isolation of Cloned DNAs that Contain a Specific Gene", Proc. Natl. Acad. Sci. 272, pp. 3961-3965.
Salton et al., 1951, "Studies of the Bacterial Cell Wall", Biochimicaet Biophysica Acta, vol. 7, pp. 19-42.
Journal of Bacteriology, vol. 45, pp. 97-105, (1981), Mazaitis, Structure of a Naturally Occurring Plasmid with Genes for Enterotoxin Production and Drug Resistance.
Journal of General Microbiology, vol. 5, pp. 391-404, (1951), M. R. J. Salton, Adsorption of Cetytrimethylammonium Bromide by Bacteria, its Action in Releasing Cellular Constitutents and its Bactericidal Effects.
Nuclear Acids Research, vol. 7, pp. 2115-2136, (1979), Gergen, Filter Replicas and Permanent Collections of Recombinant DNA Plasmids.
Infection and Immunity, vol. 32, No. 2, pp. 661-667, (1981), Kaper et al., Molecular Characterization of Environmental and Nontoxigenic Strains of Vibrio cholerae.
Journal of Infectious Diseases, vol. 145, pp. 863-869, (1982), Moseley et al., Identification of Enterotoxigenic Escherichia coli by Colony Hybridization Using Three Enterotoxin Gene Probes.
Infection and Immunity, vol. 29, pp. 140-143, (1980), Reis et al., Transfer of a CFA/I-ST Plasmid Promoted by a Conjugative Plasmid in a Strain of Escherichia coli of Serotype O128ac:H12.
Proc. Nat. Acad. Sci., vol. 72, No. 10, pp. 3961-3965, (1975), Grunstein et al., Colony Hybridization: A Method for the Isolation of Cloned DNA's that Contain a Specific Gene.

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