Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-09-19
1986-10-14
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 7, 435805, 436530, C12Q 110, G01N 5300
Patent
active
046172653
ABSTRACT:
A plate of suitable nutrient medium and agar is inoculated with bacteria from a biological sample. Bacterial colonies are cultured on the plate and are then overlaid with a soft agar layer containing bacterial lysing agents. A removable sheet with bound first animal antibodies for cholera toxin is used to contact the overlaid lysed colonies. The sheet is then exposed to a second animal antibody to cholera toxin followed by treatment with a third animal antibody against the second animal antibody, said third animal antibody being coupled to an enzyme capable of generating a chromophoric product. The sheet is then developed by immersion in a substrate and system for the bound enzyme to produce chromophoric products at the sites of enterotoxigenic bacterial colonies.
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Board of Regents University of Texas System
Herald William J.
Marantz Sidney
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