Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1987-09-28
1989-08-01
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
436525, 436528, 436530, 436535, 436807, 435 7, 435805, 422 56, 422 60, G01N 33543, G01N 33544
Patent
active
048533351
ABSTRACT:
A sandwich immunoassay method is disclosed that is useful for the identification of an antigen in biological specimens. The method is comprised of a colloidal gold labeled binder specific for epitopes or receptors of a ligand, nonporous particulate solid phase which have antiligand covalently attached, and which said reagents are combined with the biological specimen or an extract of the biological specimen, and after an appropriate incubation, the said reactant mixture is contacted to a porous film having an exposed surface area of contact no greater than 30 mm.sup.2 to separate and concentrate the particle bound colloidal gold labeled binder from the unbound. The identification of an antigen is determined by the presence of color on the surface of the solid phase particles captured on the surface of the film. A competitive inhibition assay can be also used to identify the presence of a hapten or antigen, in which case the presence of the analyte is determined by the absence of color.
REFERENCES:
patent: 4313734 (1982-02-01), Leuvering
patent: 4373932 (1983-02-01), Gribnau
patent: 4459361 (1984-07-01), Gefter
patent: 4552839 (1985-11-01), Gould
patent: 4703017 (1987-10-01), Campbell
patent: 4745075 (1988-05-01), Hadfield
J. Imm. Methods, vol. 74, Moeremans et al, "Sensitive Visualization of Ag-Ab Reactions in Dot & Blot Immune Overlay Assays with Immunogold & Immunogold/Silver Staining", pp. 353-360, 1984.
Bernstein David
Olsen Duane A.
Krupen Karen I.
Nucker Christine M.
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