Collagenase inducing factor

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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Details

435 41, 435226, C12P 2100, C12P 100, C12N 964

Patent

active

047525789

ABSTRACT:
A method for the production of collagenase inducing factor is disclosed which comprises growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture medium at 35.degree.-38.degree. C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.

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Fogh and Trempe, "New Human Tumor Cell Lines" in Human Tumor Cell Lines In Vitro (Fogh ed.), 1975, Plenum Publ. Corp, N.Y., pp. 115-159.
Fogh et al., J. Natl. Cancer Inst., 58, 209-214, 59, 221-226, (1977).
American Type Culture Collection Catalogue of Strains II, Fourth Ed. 1983, p. 175.
Moscatelli et al., Exper. Cell Res. 156, 379-390 (1985).
Doyle et al., Biochem. Biophys. Res. Commun. 130(2), 768-773 (1985).

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