Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Cosmetic – antiperspirant – dentifrice
Reexamination Certificate
1999-11-09
2003-12-09
Padmanabhan, Sreeni (Department: 1617)
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Cosmetic, antiperspirant, dentifrice
C424S443000, C424S520000, C424S572000, C530S356000, C530S856000
Reexamination Certificate
active
06660280
ABSTRACT:
The present invention relates essentially to a collagen product containing collagen of marine origin with a low odor and preferably with improved mechanical properties, to its use in the form of cosmetic or pharmaceutical compositions or products and to the carrying out of tests in vitro, for example in the context of reconstructed tissues. The invention further relates to a process for the preparation of this collagen product containing collagen of marine origin with a low odor.
In an earlier patent application published under No. WO 93/01241, the present Applicant described the use of the unpigmented skin of fish, particularly flatfish, as a novel industrial source of collagen, the process for its extraction and the collagen and biomaterials obtained by said process.
Unpigmented marine collagen, as obtained by the Applicant's previous invention, made it possible to obtain collagen industrially at an acceptable cost by virtue of the large quantities of skins available in industry, particularly skins originating from flatfish.
However, the present inventors set themselves the new technical problem of the odor generated by collagen derived from fish, in solid form; this problem did not arise within the framework of said earlier patent application insofar as the main application concerned its use as one of the components of cosmetic or pharmaceutical compositions in which the other components, present in very large majority, masked the odor by dilution.
The present inventors also set themselves the new technical problem of the odor normally generated by collagen products containing collagen of marine origin obtained from marine animals such as fish, jellyfish, molluscs and shellfish, irrespective of the form of the collagen, including hydrolyzates thereof. In fact, it has also been possible to observe that not only collagens, irrespective of their forms, whether native or not, in the form of atelocollagen, insoluble collagen, particularly collagen fibers, soluble collagen, particularly acid-soluble collagen, or hydrolyzates thereof, have a troublesome odor.
The present inventors have also been able to observe that, especially in the context of the formation of sponges, membranes or masks of collagen products in which the concentration of collagen(s) or hydrolyzates thereof is relatively high, the odor problem becomes troublesome and severely restricts its use, particularly in the field of cosmetics or pharmaceuticals and especially as a biomaterial for topical application, particularly as a mask.
By way of a new technical problem, the present inventors also set themselves the problem of the insufficiency of the mechanical properties of solid biomaterials prepared from a collagen product of marine origin.
Thus the object of the present invention is to solve both the above-mentioned technical problems individually and preferably simultaneously, and particularly preferably without affecting or degrading the collagen(s), making it possible especially to preserve the native character of this collagen or these collagens.
According to the present invention, a solution to both these problems has unexpectedly been found which also makes it possible to obtain the collagen product with satisfactory mechanical properties, especially by virtue of the fact that, if desired, the invention makes it possible to preserve the native character of the collagen.
Thus, according to a first feature, the present invention provides, by way of a novel product, a collagen product containing collagen of marine origin obtained from marine animals such as fish, jellyfish, molluscs and shellfish, which product comprises one or more collagens or derivatives thereof, including hydrolyzates, with a low odor, at least part of said collagen or said derivatives being of marine origin and having been subjected to a deodorization treatment at any stage of the manufacture of the collagen product.
The expression “collagen product” is understood within the framework of the present invention as meaning any product which contains at least one collagen, and any derived product, including hydrolyzates. “Collagen” is understood as meaning any form of collagen, whether native or not, atelocollagen, insoluble collagen, particularly collagen fibers, soluble collagen, particularly acid-soluble collagen, and any derived product, including hydrolyzates. These hydrolyzates can be obtained chemically or enzymatically in a manner well known to those skilled in the art.
Moreover, the collagen or derivatives thereof can be in totally minor or major proportions, irrespective of its form.
Thus the collagen product according to the invention can comprise from 1 to 100% by weight of collagen(s) and derivatives thereof, including hydrolyzates thereof, preferably from 10 to 100% by weight of these products.
Within the framework of the invention, the above-mentioned deodorization treatment can be carried out initially on the collagen(s), derivatives thereof or hydrolyzates thereof, by themselves or in isolation, or on the final collagen product.
Advantageously this low-odor collagen product is obtained by means of a treatment with at least one active substance having a deodorizing effect.
Said active substance is advantageously an oxidizing substance. As the oxidizing substance it is currently preferable to use at least one sulfite, particularly a metabisulfite, or a salt of said sulfite or metabisulfite, and/or hydrogen peroxide or ozone.
An alkali metal or alkaline earth metal, preferably an alkali metal such as sodium or potassium, may be used in particular as the salt of a sulfite. It is also possible to use a partial salt of a sulfite, like metabisulfite or hydrogensulfite, with an alkali metal or alkaline earth metal as defined above.
The proportion of oxidizing substances, particularly sulfite, varies between very wide limits and will generally be between 0.001% and 20% by weight, advantageously between 0.01% and 10% by weight. A proportion which ensures an industrially effective deodorization treatment may be of the order of about 5% by weight, based on the composition containing the marine collagen to be deodorized.
In one particular embodiment of the invention, this deodorization treatment may be carried out on the collagen-containing parts of marine animals, particularly finely divided fish skins, for example fish skins which have been ground.
In another advantageous embodiment of the invention, the deodorization treatment may be carried out on collagen fibers which have been precipitated from a collagen solution obtained from fish skins in at least a first extraction step, or these two treatments may be combined.
In another advantageous embodiment, the deodorization treatment is carried out on a collagen gel or a solution of collagen hydrolyzate brought into contact with ozone, particularly at an ozone flow rate of between 25 and 50 l/hour per kg of gel, the ozone content being between 20 and 50 mg/l. This deodorization with ozone may be effected by bubbling this gas into the collagen gel or the solution of collagen hydrolyzate.
According to yet another advantageous characteristic of the invention, which is actually preferred, this collagen product has improved mechanical properties and has undergone a crosslinking treatment which initially cannot be a chemical crosslinking; this is because the latter takes place in a liquid or aqueous medium on a solid sponge of very low mechanical strength and generally culminates in a disaggregation of the collagen sponge. Thus, within the framework of the invention, it is preferable to carry out a non-chemical crosslinking, preferably a physical crosslinking and particularly preferably a crosslinking by thermal dehydration, abbreviated to TDH. In this context it is preferable to carry out a thermal dehydration under vacuum at a temperature above 60° C., advantageously of between about 110 and about 130° C. and particularly of the order of 110° C.
After a first non-chemical crosslinking has been carried out, preferably by TDH, it is then possible to strengthen the crosslinking further by p
Abdul Malak Nabil
Allard Roland
Huc Alain
Coletica
Merchant & Gould, P.C. (23552)
Padmanabhan Sreeni
Wells Lauren Q.
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