Collagen-coated polystyrene microcarrier beads

Details Collagen-coated polystyrene microcarrier beads Collagen-coated polystyrene microcarrier beads

1988-04-15

1991-02-19

Rosen, Sam

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435178, 435180, 435181, 435182, 43524024, 43524025, C12N 500

Patent

active

049943880

ABSTRACT:
A microcarrier bead system for culturing anchorage-dependent cells is formed of a polystyrene core with a coating of collagen fixed thereover. In certain embodiments, the coating is a protein, such as laminin or fibronectin. The microcarrier bead is of low density, illustratively 1.02 g/cc, and therefore requires less agitation of the nutrient media to maintain suspension. This reduced stirring causes lower shear forces to impinge upon the cells, thereby improving the attachment and proliferation of the cells being cultured. The microcarrier bead of the present invention exhibits surprising advantages with respect to cell attachment and harvesting over beads formed entirely of collagen, or of DEAE-dextran coated with collagen. During harvesting, contamination of the product resulting from dissolved collagen, particularly when proteolytic enzymes are used, is minimized. Additionally, adsorption of toxins and product by the subject microcarrier beads is minimized.

REFERENCES:
patent: 3997396 (1976-12-01), Delente
patent: 4287305 (1981-09-01), Compere et al.
patent: 4373027 (1983-02-01), Berneman et al.
patent: 4448884 (1984-05-01), Henderson
patent: 4734373 (1988-03-01), Bartal
Jacobson et al., Tissue and Cell, vol. 14, No. 1 (1982), pp. 69-83.
Biological Abstracts, vol. 75, No. 1, Jan. 1, 1983, Abstract No. 1557, Jacobson et al.
Biological Abstracts, vol. 76, No. 6, Sep. 15, 1983, Abstract No. 45711, Pierschbacher et al.
Biological Abstracts, vol. 81, No. 5, Mar. 1, 1986, Abstract No. 40846, Schwarz et al.
Biological Abstracts, vol. 77, No. 11, Jun. 1, 1984, Abstract No. 80863, McAbee et al.

Affiliated with

Also associated with

Rating

Collagen-coated polystyrene microcarrier beads does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Collagen-coated polystyrene microcarrier beads, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Collagen-coated polystyrene microcarrier beads will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1143150