Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1985-03-14
1987-04-07
Cohan, Alan
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435297, 435299, 435311, 435286, C12Q 124, C12M 302
Patent
active
046561307
ABSTRACT:
A collagen coated cell growth plate comprises a substrate coated with a storage stable coating of collagen fibrils. The method of preparing the collagen coated cell growth plates comprises dispensing biologically active collagen fibrils suspended in distilled water, onto a tissue culture dish. Thereafter, the dish containing the collagen fibril suspension is placed in a laminar flow hood provided with a sterile air stream and ultraviolet light. The fibrils sediment and adhere to the bottom of the dish, the water evaporates in the sterile air stream and is removed in the laminar flow hood exhaust, and the ultraviolet light ensures that the resulting thin layer of collagen fibrils is sterile and ready for the inoculation of living cells. The method yields a convenient precoated cell growth plate which can maintain reasonable shelf life when kept at room temperature without any significant decrease in cell growth support properties.
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Cohan Alan
Rivell John A.
Yissum Research Development Company
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