Cold-shock regulatory elements, constructs thereof, and...

Details Cold-shock regulatory elements, constructs thereof, and... Cold-shock regulatory elements, constructs thereof, and...

2000-04-19

2008-09-30

Epps-Ford, Janet L. (Department: 1633)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S023700, C536S024300, C536S024320

Reexamination Certificate

active

07429654

ABSTRACT:
Regulatory elements of the 5′UTR of cold-shock inducible genes that prolong the expression of cold-shock inducible genes under conditions that elicit the cold-shock response in a bacterium, repress the expression of the cold-shock inducible genes under physiological conditions, or enhance the translation of cold-shock inducible genes under conditions that elicit a cold-shock response in bacteria; vectors incorporating these elements; and methods for expressing proteins.

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patent: WO 99/27117 (1999-06-01), None
Goldstein et al. Major cold shock protein ofEscherichia coli. Proc. Natl. Acad. Sci. USA 87(1990), 283-287.
Li Fang et al., Transcription of cspA, the gene for the major cold-shock protein ofE. coli, is negatively regulated at 37oC by the 5′-untranslated region of its mRNA, FEMS Microbiology Letters 176 (1999) pp. 39-43.
Michael L. Sprengart et al, The Downstream Box: an efficient and independent translation initiation signal inE. coli, The EMBO Journal, vol. 15, No. 3, pp. 665-674, 1996.

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