Cold shock inducible expression and production of...

Details Cold shock inducible expression and production of... Cold shock inducible expression and production of...

2003-02-25

2009-10-20

Low, Christopher S. F. (Department: 1636)

Chemistry: analytical and immunological testing

Nuclear magnetic resonance, electron spin resonance or other...

C435S320100, C435S252300, C435S252330, C435S071200

Reexamination Certificate

active

07605000

ABSTRACT:
The present invention relates to a DNA molecule or vector and a host cell containing this DNA molecule or vector which can be used to produce a heterologous polypeptide under conditions that elicit a cold shock response in the host cell. The DNA molecule and vector include a nucleotide sequence encoding a heterologous polypeptide and a promoter and 5′-UTR from a cold shock inducible gene which directs its expression. In addition, an AT-rich sequence that enhances translation under cold shock inducible conditions is either present in the coding sequence of the heterologous polypeptide or in an additional element inserted between the coding sequence and the cold shock inducible promoter and 5′-UTR.

REFERENCES:
patent: 5395614 (1995-03-01), Knapp et al.
patent: 6294711 (2001-09-01), Meulewaeter et al.
patent: 6333191 (2001-12-01), Inouye et al.
patent: 6479260 (2002-11-01), Takayama et al.
patent: 6897042 (2005-05-01), Takayama et al.
patent: 7244588 (2007-07-01), Tomono et al.
patent: WO 92/19718 (1992-11-01), None
patent: WO 9927117 (1999-06-01), None
Yamanaka et al. Mutation analysis of the 5′ untranslated region of the cold shock cspA mRNA ofEscherichia coli. Journal of Bacteriology, vol. 181, No. 20, pp. 6284-6291, Oct. 1999.
Tanabe et al. Identification of the promoter region of hteEscherichia colimajor cold shock gene, cspA. Journal of Bacteriology, vol. 174, No. 12, pp. 3867-3873, Jun. 1992.
Etchegaray et al. Translational enhancement by an element downstream of the initiation codon inEscherichia coli. The Journal of Biological Chemistry, vol. 274, No. 15, pp. 10079-10085, Apr. 1999.
Vasina et al. Recombinant protein expression at low temperatures under the transcriptional control of the majorEscherichia colicold shock promoter cspA. Applied and Environmental Microbiology, vol. 62, No. 4, pp. 1444-1447, Apr. 1996.
Vasina et al. Scale-up and optimization of the low-temperature inducible cspA promoter system. Biotechnol. Prog. vol. 14, pp. 714-721, 1998.
Fang et al. Role of the cold-box region in the 5′-untranslated region of the cspA mRNA in its transient expression at low temperatures in Journal of Bacteriology, vol. 180, No. 1, pp. 90-95, Jan. 1998.
Mackie. Stabilization of the 3′- one-thrid ofEscherichia coliribosomal protein S20 mRNA in mutants lacking polynucleotide phosphorylase. Journal of Bacteriology, vol. 171, No. 8, pp. 4112-4120, Aug. 1989.
Phadtare and Severinov. Extended -10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription. Journal of Bacteriology, vol. 187, No. 18, pp. 6584-6589, Sep. 2005.
Fang et al. Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization. Molecular Microbiology, vol. 23, No. 2, pp. 355-364, 1997.
Xia et al. The Cold Box stem-loop proximal to the 5′-end of theEscherichia colicspA gene stabilizes its mRNA at low temperature. The Journal of Biological Chemistry, vol. 277, No. 8, pp. 6005-6011, Feb. 2002.
Iost et al. mRNAs can be stabilized by DEAD-box proteins. Nature, vol. 372, No. 6502, pp. 193-196, Nov. 1994.
Mitta, et al, “Deletion analysis of cspA ofEscherichia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction”. Molecular Microbiology. (1997). 26(2):321-335.
Mujacic, et al, “Cold-inducible cloning vectors for low-temperature protein expression inEscherichia coli: application to the production of a toxic and proteolytically sensitive fusion protein”. Gene. (1999). 238: 325-332.
Qing, et al, “Cold-shock induced high-yield protein production inEscherichia coli”. Nature Biotechnology. (Jul. 2004). 22(7):877-882.
Yamanaka et al., Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation inEscherichia coli, Journal of Bacteriology, 183(9)2808-2816 (May 2001).
Dammel et al., Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA, Genes & Development, 9:626-637 (1995).
Jones et al., Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA inEscherichia coli, Proc. Natl. Acad. Sci. USA, 93:76-80 (1996).

Affiliated with

Also associated with

Rating

Cold shock inducible expression and production of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cold shock inducible expression and production of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cold shock inducible expression and production of... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4127455