Cold mold

Details Cold mold Cold mold

2007-06-26

2007-06-26

Doerrler, William C. (Department: 3744)

Refrigeration

Storage of solidified or liquified gas

Including cryostat

C062S062000, C062S066000, C249S111000, C249S119000, C249S135000, C249S177000

Reexamination Certificate

active

10952583

ABSTRACT:
A metallic, reusable mold with one or more wells. The mold is kept within a cryostat at low temperature. The mold is removed from the cryostat and a tissue specimen is placed into a well within the mold. An embedding medium is added to the well around the tissue specimen. A pre-chilled chuck is then placed against the well opening. The mold with tissue specimen, embedding medium and chuck are returned to the cryostat and the specimen is frozen into a block. The mold and contents are removed and hot water poured against the mold releasing the block with attached chuck from the well. The chuck with attached block is then available for sectioning or other processing.

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