Cold-induced expression vector

Details Cold-induced expression vector Cold-induced expression vector

2007-07-17

2007-07-17

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S070100, C435S091400, C435S320100

Reexamination Certificate

active

10538793

ABSTRACT:
A vector having a region encoding a cold shock protein gene mRNA-origin 5′-nontranslated region, characterized in that the 5′-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

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Yamanaka et al., Mutation analysis of the 5′ untranslated region of the cold shockcspAmRNA ofEscherichia coli, Journal of Bacteriology, 6284-6291 (Oct. 1999).
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Fang et al., Transcription ofcspA, the gene for the major cold-shock protein ofEscherichia coli, is negatively regulated at 37° C by the 5′-untranslated region of its mRNA,FEMS Microbiology Letters176:39-43 (1999).
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