Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1980-07-23
1982-07-20
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435253, 435317, 435848, 435886, C12N 1500, C12P 2100, C12N 120, C12N 100
Patent
active
043406742
ABSTRACT:
Novel chemical compounds, cointegrate plasmids pUC1012 and pUC1013, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6, and plasmids pUC1015 and pUC1022 which are obtained by restructuring plasmid pUC1012, and plasmids pUC1016 and pUC1023 which are obtained by restructuring plasmid pUC1013. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4273875 (1981-06-01), Manis
Murray et al in Recombinant DNA and Genetic Experimentation, pp. 53-64, Pergamon Press, (1979).
Blattner et al, Science 196, 161-169, (1977).
Armstrong et al, Science 196, 172-174, (1977).
Highlander Sarah K.
Manis Jack J.
Martinell J.
Saliwanchik Roman
Tanenholtz Alvin E.
The Upjohn Company
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