Coherent optical analyzer

Details Coherent optical analyzer Coherent optical analyzer

1974-05-13

1976-03-30

Smith, Alfred E.

Optics: measuring and testing

Blood analysis

350162SF, 356 71, 356103, G01N 3316

Patent

active

039471233

ABSTRACT:
Cells of a given type spread among an ensemble of cells of various types are identified and, if desired, counted. Coherent light is directed toward a monolayer of cells of various types. The light scattered by the monolayer of cells is collected by a lens and the collected light is filtered by an optimum filter located in the focal plane of the lens. The optimum filter is a minimum mean-square error linear filter which suppresses light scattered by cells other than the given type of cell whereby the light scattered by the given type is enhanced. The intensity of the light passed by the optimum filter is related to the number of cells of the given type contained in the monolayer of cells. If desired, a second lens may be used to collect the filtered light and focus it toward an integrating and squaring detector.
The optimum filter may be obtained photographically. In one form, a first photographic negative of a monolayer of cells having a high percentage of cells of the given type is made at the focal plane of a transforming lens mounted so as to collect the light scattered by the monolayer; and, developed for a gamma of unity. Thereafter, a second photographic negative is made at the same focal plane of a monolayer of cells containing a normal percentage of cells of the given type using the first negative as a filter. The second negative is developed for a gamma of two. The resulting photographic plate is a realization of the desired optimum (minimum mean-square error linear) filter. Alternatively, a sandwich of two photographic plates, one positive and obtained from a monolayer of cells having a high percentage of cells of the given type and the other negative obtained from a monolayer of cells having a normal percentage of cells of the given type, is constructed to provide a physical realization of the desired optimum filter.

REFERENCES:
patent: 3503684 (1970-03-01), Preston, Jr. et al.
Caulfield, "Optical Smoothing Filters," Applied Optics, Vol. 13, No. 5, pp. 996-997, Apr. 30, 1974.
Lugt, "A Review of Optical Data-Processing Techniques" Optica Acta, Vol. 15, No. 1, pp. 1-13, Feb. 1968.
Kozma et al., "Spatial Filtering for Detection of Signals Submerged in Noise," Applied Optics, Vol. 4, No. 4, pp. 387-392, Apr. 1965.
Horner, "Optical Spatial Filtering With the Least Mean-Square-Error Filter," J. Opt. Soc. Am., Vol. 59, No. 5, pp. 553-558, May 1969.

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