Coenzyme-independent L-sorbosone dehydrogenase from Pseudomonas

Details Coenzyme-independent L-sorbosone dehydrogenase from Pseudomonas Coenzyme-independent L-sorbosone dehydrogenase from Pseudomonas

1988-12-13

1992-02-04

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435189, 435877, C12P 760, C12N 902, C12N 100

Patent

active

050859935

ABSTRACT:
A coenzyme-independent L-sorbosone dehydrogenase has been prepared from a microorganism belong to the genus Pseudomonas, which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The enzyme is membrane bound and has a molecular weight of about 47,000 kDa. The enzyme is used to carry out the conversion of L-sorbosone to 2-keto-L-gulonic acid.

REFERENCES:
patent: 3043749 (1962-07-01), Huang
patent: 3907639 (1975-09-01), Makover et al.
patent: 4902617 (1990-02-01), Fujiwara et al.
Kitamura et al., "Metabolism of L-Sorbose by Enzymes from Gluconobacter melanogenus IFO 32 93", Eur. J. Appl. Microbiol., vol. 2, pp. 1-7, 1975.
Makover et al., "New Mechanisms for the Biosynthesis and Metabolisms of 2-Keto-L-Gulonic Acid in Bacteria", Biotech. Bioeng., vol. 17, No. 10, pp. 1485-1514, 1975.

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