Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-24
2006-10-24
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S254200, C435S254110, C536S023100, C536S023200, C536S023740
Reexamination Certificate
active
07125672
ABSTRACT:
The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.
REFERENCES:
patent: 4666701 (1987-05-01), Horrobin et al.
patent: 4758592 (1988-07-01), Horrobin et al.
patent: 4826877 (1989-05-01), Stewart et al.
patent: 5057419 (1991-10-01), Martin et al.
patent: 5116871 (1992-05-01), Horrobin et al.
patent: 5443974 (1995-08-01), Hitz et al.
patent: 5968809 (1999-10-01), Knutzon et al.
patent: 5972664 (1999-10-01), Knutzon et al.
patent: 6075183 (2000-06-01), Knutzon et al.
patent: 6136574 (2000-10-01), Knutzon et al.
patent: 6677145 (2004-01-01), Mukerji et al.
patent: 2003/0196217 (2003-10-01), Mukerji et al.
patent: 2005/0266537 (2005-12-01), Chen
patent: 0005277 (1982-01-01), None
patent: WO 91/13972 (1991-09-01), None
patent: WO 93/11245 (1993-06-01), None
patent: WO 94/11516 (1994-05-01), None
patent: WO 00/12720 (2000-03-01), None
patent: WO 02/090493 (2002-11-01), None
patent: WO 03/099216 (2003-12-01), None
Dyerberg, J. et al., Fatty Acid Composition of the plasma lipids in Greenland Eskimos, Amer. J. Clin Nutr. 28: pp. 958-966, 1975.
Dyerberg, J. et al., Eicosapentaenoic Acid and Prevention of Thrombosis and Atherosclerosis?, Lancet 2(8081): pp. 117-119, Jul. 15, 1978.
Shimokawa, H., Beneficial Effects of Eicosapentaenoic Acid on Endothelial Vasodilator Functions in Animals and Humans, World Rev. Nutr. Diet, 88: pp. 100-108, 2001.
Von Schacky et al.,Fatty Acids from Eskimos to Clical Cardiology—What Took Us So Long?, World Rev. Nutr. Diet, 88: pp. 90-99, 2001.
Domergue et al., Cloning and functional characterization of Phaeodactylum tricomutuim front-end desaturases involved in eicosapentaenoic acid biosynthesis, Eur. J. Biochem. 269: 4105-4113, 2002.
Beaudoin et al., Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway, Proc. Natl. Acad. Sci. U.S.A. 97(12): 6421-6, 2000.
Dyer et al.,Metabolic engineering ofSaccharomyces cerevisiaefor production of novel lipid compounds, Appl. Eniv. Microbiol., 59: pp. 224-230, 2002.
Ratledge, Microbial Oils and Fats: An Assessment of their Commercial Potential, C., Prog. Ind. Microbiol. 16: 119-206, 1982.
Brenner et al., Regulatory function of Delta6 Desaturase—Key Enzyme of Polyunsaturated Fatty Acid Synthesis, Adv. Exp. Med. Biol. 83: pp. 85-101, 1976.
Horrobin et al., Fatty acid metabolism in health and sisease: the role of delta-6-desaturase Am. J. Clin. Nutr. 57, (Suppl.) 732S-737S, 1993.
Accession No. AF465281, Mortierella alpina, Feb. 4, 2002.
Accession No. AX464731, Mortierella alpina, Jul. 16, 2002.
Picataggio Stephen K.
Zhu Quinn Qun
E. I. du Pont de Nemours and Company
Guzo David
Joike Michele
LandOfFree
Codon-optimized genes for the production of polyunsaturated... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Codon-optimized genes for the production of polyunsaturated..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Codon-optimized genes for the production of polyunsaturated... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3716891