Coating composition for culturing animal cells and method for cu

Drug – bio-affecting and body treating compositions – Solid synthetic organic polymer as designated organic active... – Aftertreated polymer

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424 7818, 424 7822, 424487, 435 703, 435402, 435399, 435396, 435395, A61K 3174

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active

056435614

ABSTRACT:
Disclosed is a coating compositions for culturing animal adhesive cells comprising a water-insoluble polymer dissolved in a lower alcohol or an aqueous lower alcohol which enable to enhance the adhesive ability and growth of the adhesive cells. Also disclosed is serum-free cell culturing method using culture vessels or carrier coated with the water-insoluble polymer having cell adhesive activity on at least a part of the surface which enable to not only culture but also subculture a variety of adhesive cells including vascular endothelial cell under serum-free condition.

REFERENCES:
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patent: 5183804 (1993-02-01), Saiki et al.
patent: 5196190 (1993-03-01), Nangia et al.
patent: 5219895 (1993-06-01), Kelman et al.
Jun. 1990 EP-A-0 373 626 (Mitsubishi Kasei Corporation).
Nov. 1990 WO-A-90 14417 (Synbiotics Corporation).
Apr. 1991 WO-A-91 05036 (Board of Regents, The University of Texas).

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