Coating agents for cell recovery

Details Coating agents for cell recovery Coating agents for cell recovery

1993-04-27

1994-06-28

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 29, 435296, 422102, 436810, A01N 102, C12Q 102, C12M 124

Patent

active

053246292

ABSTRACT:
A method for improving recovery of cells from liquid suspension by centrifugation. The method comprises coating the interior of centrifuge containers with a solution comprising an amphipathic compound prior to introduction of the cell suspension and centrifugation. The method is particularly suited for recovery or concentration of rare cells from dilute suspensions, for example when rare cells are isolated from a sample by sorting on a flow cytometer.

REFERENCES:
patent: 4865733 (1989-09-01), Tsuru et al.
patent: 4927750 (1990-05-01), Dorn
Meck, R. A., et al., "Cytometry," vol. 1 (1), 1980, pp. 84-86.

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