Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus
Patent
1995-06-02
1996-06-11
Redding, David A.
Chemistry: molecular biology and microbiology
Apparatus
Mutation or genetic engineering apparatus
935 85, 89 114, C12M 100
Patent
active
055255108
ABSTRACT:
An apparatus is disclosed for the genetic transformation of organisms by accelerated particle mediated transformation. Foreign genes are introduced into cells by coating on carrier particles which are physically accelerated into the cells by positioning the carrier particles on the external surface of a carrier ribbon which is wound on a cartridge, the carrier ribbon having an exposed portion. The carrier particles on the exposed portion of the ribbon are displaced and accelerated toward an exit port by a high pressure stream of helium gas. By rotating the ribbon, a continuous supply of carrier particles can be produced and hence large target areas can be transformed without the need to replace the particle carrier. Near the exit port, the gaseous stream is diverted through use of the Coanda effect to divert the gas stream away from a target area. The carrier particles, being much heavier than the gas, continue toward and into the target cells. The treated cells are recovered and a portion of the them contain the foreign gene in their genome.
REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5015580 (1991-05-01), Christou et al.
patent: 5066587 (1991-11-01), Jones et al.
patent: 5120657 (1992-06-01), McCabe et al.
patent: 5149655 (1992-09-01), McCabe et al.
patent: 5204253 (1993-04-01), Sanford et al.
patent: 5240855 (1993-08-01), Tomes
patent: 5405779 (1995-04-01), McCabe et al.
Klein, T. M., et al., "Particle Gun Technology: A Novel Method for the Introduction of DNA into Living Cells," Abstract for Post 28 in Biotechnology in Plant Science: Relevance to Agriculture in the Eighties, International Symposium, Ithaca, NY (1985).
Klein, T. M., et al., "High Velocity Microprojectiles for Delivering Nucleic Acids into Living Cells," Nature, 327:70-73 (1987).
Klein, T. M., et al., "Stable Genetic Transformation of Intact Nicotiana Cells by the Particle Bombardment Process," Proc. Natl., Acad. Sci. USA, 85:8502-8505 (1988).
McCabe, D. E., et al., "Stable Transformation of Soybean (Glycine max) by Particle Acceleration," Bio/Technology, 6:923-926 (1988).
Sanford, J. C., "The Biolistic Process," TIBTECH, 6:299-302 (1988).
Sanford, J. C., et al., "Delivery of Substances into Cells and Tissues Using a Particle Bombardment Process," Particulate Science and Techn., 5:27-38 (1987).
McCabe Dennis E.
Swain William F.
Agracetus, Inc.
Redding David A.
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