Coamplification of target nucleic acids using volume exclusion a

Details Coamplification of target nucleic acids using volume exclusion a Coamplification of target nucleic acids using volume exclusion a

1994-09-15

1998-01-06

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 5, 536 231, 536 243, 536 2432, 536 2433, C12P 1934, C12Q 168, C12Q 170, C07H 2104

Patent

active

057053669

ABSTRACT:
Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are used. In this manner, amplification efficiency of a given target nucleic acid can be more readily manipulated.

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