Co-integrate plasmids and their construction from plasmids of Es

Details Co-integrate plasmids and their construction from plasmids of Es Co-integrate plasmids and their construction from plasmids of Es

1982-01-26

1982-07-06

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435253, 435317, 435848, 435886, C12N 1500, C12P 2100, C12N 120, C12N 100

Patent

active

043384005

ABSTRACT:
Novel chemical compounds, recombinant plasmids pUC1026 and pUC1027, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6. These plasmids are produced by a novel process which can be used to stabilize unstable potential plasmid vectors. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin. The stabilization process disclosed herein can be used to make other stable plasmids.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4273875 (1981-06-01), Manis
Thompson, C. J., et al., "DNA cloning in Streptomyces: resistance genes from antibotic-producing species." Nature 286, 525-527.
Suarez, J. E. et al. "DNA cloning in Streptomyces. . . " Nature 286, 527-529.
Bibb, M. et al. "A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces." Nature 284, 526-531.
Murray et al. in Recombinant DNA and Genetic Experimentation, Morga et al., (ed.), Pergamon Press (1979), pp. 53-64.
Blattner et al., Science 196, 161 (1977).
Armstrong et al., Science 196, 172 (1977).
Bolivar, Gene, 4, 121 (1978).

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