Co-expression of proteins

Details Co-expression of proteins Co-expression of proteins

1997-05-30

1999-11-23

Smith, Lynette R. F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

536 236, 435 691, 4353201, 435410, 435419, 800284, 800295, 8003201, C12N 504, C12N 1529, A01H 500, A01H 510

Patent

active

059903848

ABSTRACT:
The subject invention pertains to materials and methods for transformed plants and plant tissues that are capable of expressing high levels of stable proteins which are localized as protein bodies within the plant cell. Transformed plants co-expressing high levels of both the 15 kD and 10 kD zein proteins are disclosed which accumulate to high levels as protein bodies in the vegetative tissue of the plant. Transformed plants co-expressing the 15 kD and 10 kD zein proteins are useful for providing forage crops containing increased levels of sulfur containing amino acids, such as methionine, in the diet of animals that normally feed on such crops. Also contemplated by the subject invention are transformed plants or plant tissue comprising stable protein bodies which contain heterologous proteinaceous material. In one embodiment, a stable protein body is expressed in a plant or plant tissue as a fusion protein comprising a zein protein and an operably linked protein or peptide. The protein bodies provided in the present invention are resistant to rumin digestion or environmental degradation.

REFERENCES:
Matzke and Matzke. Plant Physiol. 1995. vol. 107: 679-685.
Finnegan and MCElory. Bio/Technology. 1994. 12: 883-888.
Ohtani et al. Plant Molecular Biology. 1990. vol. 16: 117-128.
Giannazza et al. Phytochemistry. 1977. vol. 16: 315-317.
Bagga et al. Plant Physiol. 1995. vol. 107: 13-23. January issue.
Bray et al. Planta. 1987. vol. 172: 364-370.
Kirhara et al. Molecular and general Genetics. 1988. vol. 211: 477-484.
Marks and Larkins. Journal of Biological Chemistry. 1982. vol. 257:9976-9983).
Abe, S. et al. (1991) "Protein bodies in corn endosperm are enclosed by and enmeshed in F.-actin." Protoplasma 165: 139-149.
Maliga, P. (1978) "Resistant mutant and their use in genetic manipulation." Frontiers of Plant Tissue Culture (Ed: Thorpe TA) pp. 381-392.
Argo, P., et al. (1982) "A Structural Model for Maize Zein Proteins" The Journal of Biological Chemistry, vol. 257, No. 17, 9984-9990.
Altenbach, S.B., et al. (1992) "Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine" Plant Molecular Biology 18: 235-245.
Barnes, D.K., et al. (1988) "Highlights in the USA and Canada" Agronomy Monograph No. 29: 1-24.
Boston, R.S., et al. (1991) "Increased Expression of the Maize Immunoglobulin Binding Protein Homolog b-70 in Three Zein Regulatory Mutants" The Plant Cell, vol. 3, 497-505.
De Clercq, A., et al. (1990) "Expression and Processing of an Arabidopsis 2S Albumin in Transgenic Tobacco" Plant Physiol. 92: 899-907.
Esen, A., et al. (1992) "Immunocytochemical Localization of .delta.-Zein In The Protein Bodies of Maize Endosperm Cells" American Journal of Botany 79(3): 243-248.
Gianazza, E., et al. (1977) "Amino Acid Composition of Zein Molecular Components" Physiochemistry, vol. 16, 315-317.
Guerche, P., et al. (1990) "Expression of the 2S albumin from Bertholletia excelsa in Brassica napus" Mol Gen Genet 221: 306-314.
Hoffman, L.M., et al. (1988) "A modified storage protein is synthesized, processed, and degraded in the seeds of transgenic plants" Plant Molecular Biology 11:717-729.
Kaldy, M.S., et al. (1979) "Amino acid composition of sainfoin forage" Grass and Forage Science, vol. 34, 145-148.
Karchi, H., et al. (1993) "Seed-specific expression of a bacterial desensitized aspartate kinase increases the production of seed threonine and methionine in transgenic tobacco" The Plant Journal, 3(5): 721-727.
Kirihara, J.A., et al. (1988) "Differential expression of a gene for a methionine-rich storage protein in maize" Mol Gen Genet 211: 477-484.
Langridge, P., et al. (1983) "A Zein Gene of Maize is Transcribed from Two Widely Separated Promoter Regions" Cell, vol. 34, 1015-1022.
Larkins, B.A., et al. (1989) "Zein Gene Expression during Maize Endosperm Development" The Molecular Basis of Plant Development, pp. 109-120.
Larkins, B.A., et al. (1978) "Synthesis and Deposition of Zein in Protein Bodies of Maize Endosperm" Plant Physiol. 62: 256-263.
Lee, K.H., et al. (1976) "Genetic Regulation of Storage Protein Content in Maize Endosperm" Biochemical Genetics, vol. 14: 641-650.
Lending, C.R., et al. (1989) "Changes in the Zein Composition of Protein Bodies during Maize Endosperm Development" The Plant Cell, vol. 1: 1011-1023.
Li, X., et al. (1993) "Rice Prolamine Protein Body Biogenesis A BiP-Mediated Process" Science, vol. 262, pp. 1054-1056.
Michaud, R., et al. (1988) "World Distribution and Historical Development", Alfalfa and Alfalfa Improvement--Agronomy Monograph No. 29: 25-91.
Misra, P.S., et al. (1972) "Endosperm Protein Systhesis in Maize Mutants with Increased Lysine Content" Science, vol. 176: 1425-1427.
Ohtani, T., et al. (1991) "Normal and lysine-containing zeins are unstable in transgenic tobacco seeds", Plant Molecular Biology 16: 117-128.
Pelham, H.R.B. (1990) "The retention signal for soluble proteins of the endoplasmic reticulum", Elsevier Science Publishers 0376-5067/90 pp. 483-486.
Phillips, R.L., et al. (1985) "Elevated Protein-Bound Methionine in Seeds of a Maize Line Resistant to Lysine Plus Threonine", American Association of Cereal Chemists, vol. 62, No. 3: 213-218.
Reish, B. et al. (1981) "Selection and Characterization of Ethionine-resistant Alfalfa (Medicago sativa L.) Cell Lines" Theor. Appl. Genet, 59: 89-94.
Saalbach, I., et al. (1994) "A chimeric gene encoding the methionine-rich 2S albumin of the Brazil nut (Bertholletia excelsa H.B.K.) Is stably expressed and inherited in transgenic grain legumes" Mol. Gen. Genet 242: 226-236.
Schroeder, H.E., et al. (1991) "Expression of a Chicken Ovalbumin Gene in Three Lucerne Cultivars" Aust. J. Plant Physiol. 18: 495-505.
Wallace, J.C., et al. (1988) "Aggregation of Lysine-Containing Zeins into Protein Bodies in Xenopus Oocytes" Science, vol. 240: 662-664.
Williamson, J.D., et al. (1988) "The Synthesis of a 19 Kilodalton Zein Protein in Transgenic Petunia Plants" Plant Physiol. 88: 1002-1007.
Zhang, F., et al. (1992) "Increases in binding protein (BiP) accompany changes in protein body morphology in three high-lysine mutants of maize" Protoplasma 171: 142-152.

Affiliated with

Also associated with

Rating

Co-expression of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Co-expression of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Co-expression of proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1223840