Co-culture production of thermostable enzymers and ethanol

Details Co-culture production of thermostable enzymers and ethanol Co-culture production of thermostable enzymers and ethanol

1984-09-18

1986-08-05

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Process involving micro-organisms of different genera in the...

435162, 435205, 435210, 435842, C12D 3900, C12D 714, C12N 934, C12N 944

Patent

active

046043527

ABSTRACT:
Starch is converted directly to ethanol by growing a co-culture of Clostridium thermosulfurogenes and Clostridium thermohydrosulfuricum under anaerobic conditions on a starch containing substrate in a medium comprising essential vitamins, minerals and growth factors. The co-culture also produces the thermostable enzymes .beta.-amylase, glucoamylase and pullulanase.

REFERENCES:
Zeikus, J. G., A. Ben-Bassat, and P. Hegge. 1980, Microbiology of Methanogenesis in Thermal, Volcanic Environments, J. Bact. 143: 432-440.
Schink, B., and J. G. Zeikus, 1983, Clostridium thermosulfurogenes sp. nov., A New Thermophile That Produces Elemental Sulfur From Thiosulphate. J. Gen. Microbiol. 129: 1149-1158.
Hyun, H. H., J. G. Zeikus, R. Longin, J. Millet, and A. Ryter, 1983, Ultrastructure and Extreme Heat Resistance of Spores from Thermophilic Clostridia, J. Bact. 156: 1332-1337.
Matteuzzi, D., F. Hollaus, and B. Biavati, 1978, Proposal of neotype for Clostridium thermohydrosulfuricum and the merging of Clostridium tartarivorum with Clostridium thermosaccharolyticum. Int. J. System, Bacteriol, 28: 528-531.
Lovitt, R. W., R. Longin and J. G. Zeikus, 1984, Ethanol Production by Thermophilic Bacteria: Physiological Comparison of Solvent Effects on parent and alcohol-tolerant strains of Clostridium thermohydrosulfuricum, Appl. Environ. Microbiol, 48: 171-177.
Zeikus, J. G. 1983, Metabolic Communication Between Biodegradative Populations in Nature, In: Microbs in their natural environments, J. H. Slater, R. Whittenbury and J. W. T. Wimpenny (Eds.) Symposium 34 Society for General Microbiology Ltd. Cambridge University Press 1983.
Zeikus, J. G. and T. K. Ng. 1982, Thermophilic Saccharide Fermentations In: Annual Report of Fermentation Processes. G. Tsao, Editor, vol. 5, 7: 263-289.
Ng. T. K., A. Ben-Bassat, and J. G. Zeikus, 1981, Ethanol Production by Thermophilic Bacteria: Fermentation of Cellulosic Substrates by co-cultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum Appl. Environ. Microbiol. 41: 1337-1343.
Zeikus, J. G. 1979, Thermophilic Bacteria: Ecology, Physiology, and technology, Enzyme Microb. Technol. 1: 243-252.

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