Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2006-03-28
2006-03-28
Lankford, Jr., Leon B. (Department: 1651)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S344000, C435S183000, C435S816000, C210S767000, C210S511000
Reexamination Certificate
active
07019120
ABSTRACT:
A method of extracting a polypeptide of interest from a fermentation broth comprising:i) adjusting the pH close to pI of the polypeptide of interest;ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower;iii) cooling the mixture for solubilization and incubating at above cloud point for extraction;iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; andv) recovering the surfactant-rich top phase containing the polypeptide of interest.
REFERENCES:
patent: 6105786 (2000-08-01), Braunstein et al.
Terstappen et al, Journal of Biotechnology, 1993, vol. 28, p. 263-275.
Azzabi Fahd
Iyer Prashant
Rane Kishore
Wenger Kevin S.
Ford Allison M.
Garbell Jason I.
Lambins Elias J.
Lankford , Jr. Leon B.
Novozymes A/S
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