Clostridium perfingens type a enterotoxin toxoid and methods of

Details Clostridium perfingens type a enterotoxin toxoid and methods of Clostridium perfingens type a enterotoxin toxoid and methods of

1994-03-15

1997-12-09

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 711, 435 911, 4352523, 43525233, 536 231, C12P 2102

Patent

active

056959567

ABSTRACT:
Escherichia coli strains that produce recombinant Clostridium perfringens type A enterotoxin toxoids from a Clostridium perfringens type A enterotoxin gene fragment encoding the Clostridium perfringens type A enterotoxin binding domain subcloned into an expression vector for forming plasmids are disclosed. The Clostridium perfringens type A enterotoxin toxoids of this invention recognize, irreversibly bind to and saturate receptor sites on intestinal membranes and, thus effectively compete for these receptor sites with Clostridium perfringens type A enterotoxin. The toxoids of this invention may be used to treat the symptoms of Clostridium perfringens food poisoning in patients. Vaccines are also disclosed that may be used to prevent the symptoms of Clostridium perfringens food poisoning in patients. Processes for preparing the plasmids and toxoids of this invention and for using the toxoids and vaccines of this invention are also provided.

REFERENCES:
patent: 4264588 (1981-04-01), Orcutt
patent: 4292307 (1981-09-01), Zemlyakova
patent: 4689299 (1987-08-01), Insel et al.
"The 31 C-Term Amino Acids of Clostridium perfringens Enterotoxin Defines e Receptor Binding Domain", poster displayed at Annual Meeting of American Society for Microbiology, Anaheim, California (May 1990), P. C. Hanna and B. A. McClane Abstract.
"A recombinant C-terminal toxin fragment provides evidence that membrane insertion is important for Clostridium perfringens enterotoxin cytotoxicity", Molecular Biology, vol. 5, No. 1, pp. 225-230 (Jan. 1991),Philip C. Hanna and Bruce A. McClane.
"Localization of the Receptor-binding Region of Clostridium perfringens Enterotoxin Utilizing Cloned Toxin Fragments and Synthetic Peptides", Journal of Biological Chemistry, vol. 266, No. 17, pp. 11037-11043 (Jun. 1991), Philip C. Hanna, Timothy A. Mietzner, Gary K. Schoolnik and Bruce A. McClane.
"Isolation and Function of a Clostridium perfringens Enterotoxin Fragment" Infection and Immunity, vol. 55, No. 12, pp. 2912-2915 (Dec. 1987), Yas uhiko Horiguchi, Tetsuo Akai, and Genji Sakaguchi.
"Molecular Cloning of the 3' Half of the Clostridium perfringens Enterotoxin Gene and Demonstration that This Region Encodes Receptor-Binding Activity", Journal of Bacteriology, vol. 171, No. 12, pp. 6815-6820 (Dec. 1989) Philip C. Hanna, Andrew P. Wnek, and Bruce A. McClane.
Van Damme-Jongsten et al., Antonic van Leeuwenhoek, 56(2): 181-190, 1989.
Amicon, Centricon Microconcentrators Operating Instructions.
Crowl, et al Gene 38:31-38 1985.
McClane et al J Clin Micro 19(2):112-115 1984.
Popoff FEMS Micro Lett 21:1-5 1984.
Current Protocols in Molecular Biology Wiley & Sons Publisher 1987 chapters 1-3 & 6.
Hanna, et al J. Bacteriology 171(12) 6815-6820 1989.
Van Damme-Jongsten, et al., 1990, Snythetic DNA Probes for Detection of Enterotoxigenic Clostridium perfingens Strains Isolated from Outbreaks of Food Poisoning, J. Clin. Micro. 28 (1): 131-133.
Van Damme-Jongsten, et al., 1990, Testing strains of Clostridium perfringens type A isolted from diarrhoeic piglets for the presence of the enterotoxin gene, Veterinary record 126: 191-192.
Iwanejko, et al., 1989 Cloning in Escherichia coli of the Enterotoxin Gene from Clostridium perfringens Type A, J. Gen. Microbiology 135: 903-909.
Granum, 1986, Structure and Mechanism of Action of the Enterotoxin from Clostridium Perfringes. In: Falmagney, P, et al, ed. Second European Workshop on bacterial protein toxins, 327-343.
Richardson & Granum, 1985, The amino acid sequence of the enterotoxin from Clostridium perfringes type A, FEBS Letters 182 (2): 479-484.
Ricardson & Granum, 1983, sequence of the Amino-Terminal Part of Enterotoxin from Clostridium perfringes Type A Identification of Points of Trypsin Activation, Infection and Immunity 40 (3): 943-949.
Granum, 1982, Inhibition of Proteins Synthesis by a Tryptic Polypeptide of Clostridium Perfringes Type A Enterotoxin, Biochim. Biophys. Acta. 708: 6-11.
Granum, et al., 1981, Tryspin Activation of Enterotoxin from Clostridium Perfringesn Type A, Biochim. Biophys. Acta. 668: 325-332.

Affiliated with

Also associated with

Rating

Clostridium perfingens type a enterotoxin toxoid and methods of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Clostridium perfingens type a enterotoxin toxoid and methods of , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Clostridium perfingens type a enterotoxin toxoid and methods of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1606795