Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2011-08-09
2011-08-09
Liu, Sue (Department: 1653)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S007210, C435S173100
Reexamination Certificate
active
07993906
ABSTRACT:
The present invention provides a sensitive system for measuring the physiological response of an in-vitro cell culture to an environmental parameter. An electrical property of the cell culture is measured as a control signal, and a parameter of a stimulus is adjusted in real time to maintain the control signal at a specified value as the environment of the cell culture is altered, for example, pharmacologically. Artifact reduction and real-time control methods are two key aspects of preferred embodiments of the invention, and enable highly accurate determination of pulse parameters which elicit a desired response. Both aspects must be highly robust to the natural variations inherent in a biological system. This system is beneficial for studying the effects of environmental alterations because extremely small changes in the physiological response can be measured over time, revealing the magnitude and time-dependence of the impact of these alterations on the cell culture.
REFERENCES:
patent: 4750499 (1988-06-01), Hoffer
patent: 5916239 (1999-06-01), Geddes et al.
patent: 5981268 (1999-11-01), Kovacs et al.
patent: 6114164 (2000-09-01), Dennis et al.
patent: 6135978 (2000-10-01), Houben et al.
patent: 6819956 (2004-11-01), DiLorenzo
patent: 7294333 (2007-11-01), Feld et al.
Doerr T. et al. Ionic currents contributing to the action potential in single ventricular myocytes of the guinea pig studied with action potential clamp, Pflugers Archiv Europian Journal of Physiology, 1990, vol. 416, pp. 230-237, entire document.
DeHaan R.L. et al. Membrane response to current pulses in spheroidal aggregates of embryonic heart cells, The Journal of General Physiology, Feb. 1, 1975, vol. 65, pp. 207-222, entire document.
Whittington R.H. et al. “A Multi-Parameter, Feedback-Based Electrical Stimulation System for Cardiomyocyte Cultures”, Transducers '03; Downloaded from IEEE Xplore, The 12th International Conference an Solid State Sensors, Actuators and MicroSystems. Boston, Jun. 8-12. 2003, pp. 983-986.
Solberg J. “Closed-Loop Control of Functional Electrical Stimulation for Human Gait: Introduction, Feedback Sensors, and Foreseeable Difficulties”, A research report submitted for fulfilment of ELEC 8900 (full-time project) to the School of Electrical and Information Engineering, The University of Sydney on Nov. 15, 2000, total pp. 1-63.
Daniel A. Wagenaar et al., “A versatile all-channel stimulator for electrode arrays, with real-time control,” J. Neural Eng. 1 (2004) 39-45.
Steve M. Potter et al., “Long-Term Bidirectional Neuron Interfaces for Robotic Control, and In Vitro Learning Studies,” Proceedings of the 25thAnnual International Conference of the IEEE EMBS pp. 3690-3693, 2003.
Kovacs Gregory T. A.
Whittington R. Hollis
Liu Sue
Lumen Patent Firm
Singh Satyendra
The Board of Trustees of the Leland Stanford Junior University
LandOfFree
Closed-loop electrical stimulation system for cell cultures does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Closed-loop electrical stimulation system for cell cultures, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Closed-loop electrical stimulation system for cell cultures will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2759507